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plasmid dna pbluescript ii sk  (Qiagen)


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    Qiagen plasmid dna pbluescript ii sk
    Plasmid Dna Pbluescript Ii Sk, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+dnas+pbluescript+ii+sk/plasmid+pbluescript/pm37209567-51-1-18
    Average 90 stars, based on 1 article reviews
    plasmid dna pbluescript ii sk - by Bioz Stars, 2026-09
    90/100 stars

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    Plasmid Preparation:

    Article Title: Strong preference of BRCA1 protein to topologically constrained non-B DNA structures.
    Article Snippet: .. Supercoiled plasmid DNAs of pBluescript II SK (−), and derived plasmids pCFNO [48], pCMYC and pTA50 were isolated from bacterial strain DH5α as described in the QIAGEN protocol (QIAGEN GmbH, Germany). .. XhoI restriction enzyme (New England Biolabs, UK) was used for linearization of plasmids. pCMYC plasmid was constructed by cloning the 141 bp EcoRI/HindIII restriction fragment of pNHE plasmid [49] into the EcoRI/HindIII site of pBSK. pTA50 plasmid was constructed by cloning of (dT)50. (dA)50 sequence, forming a DNA triplex, into the EcoRV site of pBSK.

    Article Title: Strong preference of BRCA1 protein to topologically constrained non-B DNA structures
    Article Snippet: .. Supercoiled plasmid DNAs of pBluescript II SK (−) , and derived plasmids pCFNO [ ], pCMYC and pTA50 were isolated from bacterial strain DH5α as described in the QIAGEN protocol (QIAGEN GmbH, Germany) . .. Xho I restriction enzyme (New England Biolabs, UK) was used for linearization of plasmids. pCMYC plasmid was constructed by cloning the 141 bp Eco RI/ Hind III restriction fragment of pNHE plasmid [ ] into the Eco RI/ Hind III site of pBSK. pTA50 plasmid was constructed by cloning of (dT) 50 . (dA) 50 sequence, forming a DNA triplex, into the EcoR V site of pBSK.

    Article Title: The central region of BRCA1 binds preferentially to supercoiled DNA.
    Article Snippet: BRCA1 is a multifunctional tumor suppressor protein with implications in regulating processes such as cell cycle, transcription, DNA repair, and chromatin remodeling.. The function of BRCA1 likely involves interactions with a vast number of proteins and likewise DNA.. To this date there is only fragmentary evidence about BRCA1 binding to DNA.

    Derivative Assay:

    Article Title: Strong preference of BRCA1 protein to topologically constrained non-B DNA structures.
    Article Snippet: .. Supercoiled plasmid DNAs of pBluescript II SK (−), and derived plasmids pCFNO [48], pCMYC and pTA50 were isolated from bacterial strain DH5α as described in the QIAGEN protocol (QIAGEN GmbH, Germany). .. XhoI restriction enzyme (New England Biolabs, UK) was used for linearization of plasmids. pCMYC plasmid was constructed by cloning the 141 bp EcoRI/HindIII restriction fragment of pNHE plasmid [49] into the EcoRI/HindIII site of pBSK. pTA50 plasmid was constructed by cloning of (dT)50. (dA)50 sequence, forming a DNA triplex, into the EcoRV site of pBSK.

    Article Title: Strong preference of BRCA1 protein to topologically constrained non-B DNA structures
    Article Snippet: .. Supercoiled plasmid DNAs of pBluescript II SK (−) , and derived plasmids pCFNO [ ], pCMYC and pTA50 were isolated from bacterial strain DH5α as described in the QIAGEN protocol (QIAGEN GmbH, Germany) . .. Xho I restriction enzyme (New England Biolabs, UK) was used for linearization of plasmids. pCMYC plasmid was constructed by cloning the 141 bp Eco RI/ Hind III restriction fragment of pNHE plasmid [ ] into the Eco RI/ Hind III site of pBSK. pTA50 plasmid was constructed by cloning of (dT) 50 . (dA) 50 sequence, forming a DNA triplex, into the EcoR V site of pBSK.

    Isolation:

    Article Title: Strong preference of BRCA1 protein to topologically constrained non-B DNA structures.
    Article Snippet: .. Supercoiled plasmid DNAs of pBluescript II SK (−), and derived plasmids pCFNO [48], pCMYC and pTA50 were isolated from bacterial strain DH5α as described in the QIAGEN protocol (QIAGEN GmbH, Germany). .. XhoI restriction enzyme (New England Biolabs, UK) was used for linearization of plasmids. pCMYC plasmid was constructed by cloning the 141 bp EcoRI/HindIII restriction fragment of pNHE plasmid [49] into the EcoRI/HindIII site of pBSK. pTA50 plasmid was constructed by cloning of (dT)50. (dA)50 sequence, forming a DNA triplex, into the EcoRV site of pBSK.

    Article Title: Strong preference of BRCA1 protein to topologically constrained non-B DNA structures
    Article Snippet: .. Supercoiled plasmid DNAs of pBluescript II SK (−) , and derived plasmids pCFNO [ ], pCMYC and pTA50 were isolated from bacterial strain DH5α as described in the QIAGEN protocol (QIAGEN GmbH, Germany) . .. Xho I restriction enzyme (New England Biolabs, UK) was used for linearization of plasmids. pCMYC plasmid was constructed by cloning the 141 bp Eco RI/ Hind III restriction fragment of pNHE plasmid [ ] into the Eco RI/ Hind III site of pBSK. pTA50 plasmid was constructed by cloning of (dT) 50 . (dA) 50 sequence, forming a DNA triplex, into the EcoR V site of pBSK.

    Article Title: The central region of BRCA1 binds preferentially to supercoiled DNA.
    Article Snippet: BRCA1 is a multifunctional tumor suppressor protein with implications in regulating processes such as cell cycle, transcription, DNA repair, and chromatin remodeling.. The function of BRCA1 likely involves interactions with a vast number of proteins and likewise DNA.. To this date there is only fragmentary evidence about BRCA1 binding to DNA.



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    PMB had high affinity for DNA and inhibited electrophoretic migration of plasmid at higher concentrations. (A) 60 ng of pBlueScript II DNA incubated with PMB (12.5–500 μM) in a topoisomerase I buffer for 2 h at 37 °C. Samples were resolved by electrophoresis. (B) Chemical structure of PMB with potential DNA binding sites (including the Dab residues) highlighted.

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    Figure Lengend Snippet: PMB had high affinity for DNA and inhibited electrophoretic migration of plasmid at higher concentrations. (A) 60 ng of pBlueScript II DNA incubated with PMB (12.5–500 μM) in a topoisomerase I buffer for 2 h at 37 °C. Samples were resolved by electrophoresis. (B) Chemical structure of PMB with potential DNA binding sites (including the Dab residues) highlighted.

    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Techniques: Migration, Plasmid Preparation, Incubation, Electrophoresis, Binding Assay